The CANCER lab
Cancer is one of the most common causes of death worldwide. Available treatments are associated with numerous side effects and only a low percentage of patients achieve complete remission. Therefore, there is a strong need for new therapeutic strategies. In this regard, pulsed electromagnetic field (PEMF) therapy presents several potential advantages including non-invasiveness, safety, lack of toxicity for non-cancerous cells, and the possibility of being combined with other available therapies. Indeed, PEMF stimulation has already been used in the context of various cancer types including skin, breast, prostate, hepatocellular, lung, ovarian, pancreatic, bladder, thyroid, and colon cancer in vitro and in vivo.
Breast Cancer Treatment
PEMF - Pulsed Electromagnetic Field has shown promising results as an alternative treatment for individuals dealing with breast cancer. PEMF can be used with all traditional cancer medicines.
Colon Cancer Treatment
PEMF - Pulsed Electromagnetic Field has shown promising results as an alternative treatment for individuals dealing with colon cancer. PEMF can be used with all traditional cancer medicines.
Prostate Cancer Treatment
PEMF - Pulsed Electromagnetic Field has shown promising results as an alternative treatment for individuals dealing with prostate cancer. PEMF can be used with all traditional cancer medicines.
Lung Cancer Treatment
PEMF - Pulsed Electromagnetic Field has shown promising results as an alternative treatment for individuals dealing with lung cancer. PEMF can be used with all traditional cancer medicines.
Pancreatic Cancer Treatment
PEMF - Pulsed Electromagnetic Field has shown promising results as an alternative treatment for individuals dealing with pancreatic cancer. PEMF can be used with all traditional cancer medicines.
"Character is revealed,
when the pressure is applied"
Cancer is one of the most common causes of death worldwide and accounted for 8.2 million deaths in 2012. The number of cancer-related deaths is predicted to increase to over 11 million by 2030. The types of cancer with the highest incidence are lung (1.59 million people), liver (745,000), stomach (723,000), colon and rectum (694,000), breast (521,000), and esophagus (400,000).
In oncology, the selection of correct treatment strategy, in early disease stages, is crucial to increase the probability of remission and improve survival. Available cancer treatments include chemotherapy, immunotherapy or antibody-based therapy, radiation therapy, and surgery. The therapeutic strategy is chosen taking into account the individual patient's medical assessment, type of cancer, location, and disease stage. Multi-modal treatments are often required to reduce the therapy-induced side effects related to pharmacological as well as other approaches including surgery. Chemotherapy-induced side effects depend on various variables such as the drug employed, its dosage, and treatment duration. These side effects include pain, fatigue, throat and mouth sores, diarrhea, nausea, vomiting, constipation, and blood disorders. Side effects affecting the nervous system are commonly experienced with chemotherapy and include cognitive dysfunction, headache, dizziness, vision loss and vision disturbances such as blurred or double vision, changes in learning and memory, sexual dysfunction, ataxia, and peripheral neuropathy.
Rashes, fever, hypotension, colitis or other gastrointestinal problems, and thyroid dysfunctions are immunotherapy-related side effects. The main radiotherapy-induced side effects are dry mouth and gum sores, jaw stiffness, nausea, lymphedema, swallowing difficulties, shortness of breath, breast or nipple soreness, rectal bleeding, incontinence, bladder irritation, and pituitary dysfunction. Surgical techniques, such as minimally invasive surgery, also result in pain, fatigue, appetite loss, swelling and bruising around the site of surgery, bleeding, infection, lymphedema, and organ dysfunction.
Numerous studies support the development of new treatments in oncology to be added to the traditional protocols to increase the effectiveness of available treatments, reducing side effect profile, and the patients' quality of life. Such resources include traditional Chinese medicine, Ayurvedic medicine, homeopathy, and naturopathy. While complementary and alternative medicine (CAM) is not generally considered part of conventional medicine, it has been widely used in the oncology field as an add-on therapy to control patients' symptoms and improve their quality of life.
The beginning of the 20th century saw the first therapeutic applications of CAM therapies for cancer treatment; these therapies include acupuncture, chromotherapy, therapeutic touch (reiki), and pulsed electromagnetic field (PEMF) therapy. In this review, we have focused on PEMF therapy, a noninvasive technique characterized by electromagnetic fields inducing microcurrents to the entire body or locally to target specific body tissues. Exposure to PEMFs in the 0–300 Hz range is a therapeutic tool extensively used for the treatment of several pathologies including osteoarthritis, Parkinson's disease, postsurgical pain and edema, treatment of chronic wounds, and facilitation of vasodilatation and angiogenesis producing direct stimulation to excitable cells including nerve and muscle cells. Stimulation with sufficient intensity and duration induces a current across targeted cell membranes, activating nerve cells or muscles to propagate action potentials. Indeed, PEMF therapy can be used as an adjuvant treatment to chemotherapy and radiotherapy with the aim of reducing their dosage, mitigating any harmful secondary side effects, and enhancing patient's prognosis.
PEMF therapy has been extensively studied in vitro using various human cancer cell lines, such as pheochromocytoma-derived (PC12), breast cancer (e.g., MCF7, MDA-MB-231 and T47D), and colon cancer (SW-480 and HCT-116). These studies have shown that PEMF therapy may exert proliferative inhibition and mitotic spindle disruption, block the development of neovascularization required for tumor supply and exacerbate an inherent or induced genetic instability by reducing the stringency of the late-cycle (G2) checkpoint. While chemotherapy is not specific to cancer cells and targets all rapidly dividing cells, PEMFs exert selective cytotoxic effect on neoplastic cells making this therapy a highly promising strategy.
BREAST CANCER TREATMENT
BREAST CANCER AND PEMF TREATMENT
Daily PEMF was found to retard angiogenesis and growth of a human breast cancer xenograph causing the tumor to develop proportionately larger areas of necrosis and hypoxia and smaller areas of proliferatively active cancer cells.
Pulsed Electromagnetic Field Therapy, abbreviated PEMF, is a very simple, painless, and non-invasive way to address abnormal resonance in cancer cells. In addition, PEMF has been shown to assist in making cell membranes more permeable.
Introduction
A common drawback of many anticancer therapies is non-specificity in action of killing. We investigated the potential of ultra-low intensity and frequency pulsed electromagnetic fields (PEMFs) to kill breast cancer cells. Our criteria to accept this technology as a potentially valid therapeutic approach were: 1) cytotoxicity to breast cancer cells and; 2) that the designed fields proved innocuous to healthy cell classes that would be exposed to the PEMFs during clinical treatment.
METHODS
MCF7 breast cancer cells and their normal counterparts, MCF10 cells, were exposed to PEMFs and cytotoxic indices measured in order to design PEMF paradigms that best kill breast cancer cells. The PEMF parameters tested were: 1) frequencies ranging from 20 to 50 Hz; 2) intensities ranging from 2 mT to 5 mT and; 3) exposure durations ranging from 30 to 90 minutes per day for up to three days to determine the optimum parameters for selective cancer cell killing.
RESULTS
We observed a discrete window of vulnerability of MCF7 cells to PEMFs of 20 Hz frequency, 3 mT magnitude and exposure duration of 60 minutes per day. The cell damage accrued in response to PEMFs increased with time and gained significance after three days of consecutive daily exposure. By contrast, the PEMFs parameters determined to be most cytotoxic to breast cancer MCF-7 cells were not damaging to normal MCF-10 cells.
CONCLUSION
Based on our data it appears that PEMF-based anticancer strategies may represent a new therapeutic approach to treat breast cancer without affecting normal tissues in a manner that is non-invasive and can be potentially combined with existing anti-cancer treatments.
COMPLETE STUDY
Colon CANCER TREATMENT
COLON CANCER AND PEMF TREATMENT
Indeed, PEMF stimulation has already been used in the context of various cancer types including skin, breast, prostate, hepatocellular, lung, ovarian, pancreatic, bladder, thyroid, and colon cancer in vitro and in vivo. At present, only limited application of PEMF in cancer has been documented in humans.
Pulsed Electromagnetic Field Therapy, abbreviated PEMF, is a very simple, painless, and non-invasive way to address abnormal resonance in cancer cells. In addition, PEMF has been shown to assist in making cell membranes more permeable.
ABSTRACT
Pulsed Electromagnetic Field in colon cancer cell lines, in particular, HCT 116. Two 96-well plates were prepared to place two groups (i.e. control group and experimental group) of colon cancer cell lines to monitor the effects of PEMF by performing cytotoxicity test thru Alamar Blue dye. The frequencies used in the experiment were derived from the resonant length of the DNA base pair size of the aberrant chromosomes of HCT 116 – chromosome 10q26, chromosome 16p13.3, chromosome 18p11.2. The researchers also attempted to construct a PEMF machine with a 3.3MHz carrier frequency and RF (radio frequency) amplifier to achieve a larger power output.
The PEMF machine was tested using a digital oscilloscope and an incandescent bulb as an alternate plasma bulb. Using commercially available PEMF machine, results showed that most of the computed DNA frequencies has reduced cytotoxicity levels of the experimental group compared to the control group. This indicates that most of the cancer cells dies from the PEMF machine programmed from the calculated DNA frequencies. The p-value obtained from statistical t-test showed that most of the calculated DNA frequencies had a significant effect on the colon cancer cells since most of the values were below the alpha level (critical level) of 0.05 (95 % confidence level). Among the DNA frequencies used in the study, 656 Hz has shown the most significant effect in killing the colon cancer cell lines – HCT 116. It was shown that the we’ll known 656 Hz DNA frequency for colon cancer cell line came from the MC2R gene of chromosome 18p11.2 due to its close DNA base pair resonant length proximity with calculated upper band rife frequency at 662.84 Hz.
COMPLETE STUDY
PROSTATE CANCER TREATMENT
PROSTATE CANCER AND PEMF TREATMENT
Daily PEMF was found to retard angiogenesis and growth of a human breast cancer xenograph causing the tumor to develop proportionately larger areas of necrosis and hypoxia and smaller areas of proliferatively active cancer cells.
Pulsed Electromagnetic Field Therapy, abbreviated PEMF, is a very simple, painless, and non-invasive way to address abnormal resonance in cancer cells. In addition, PEMF has been shown to assist in making cell membranes more permeable.
ABSTRACT
Current popular cancer treatment options, include tumor surgery, chemotherapy, and hormonal treatment. These treatments are often associated with some inherent limitations. For instances, tumor surgery is not effective in mitigating metastases; the anticancer drugs used for chemotherapy can quickly spread throughout the body and is ineffective in killing metastatic cancer cells. Therefore, several drug delivery systems (DDS) have been developed to target tumor cells, and release active biomolecule at specific site to eliminate the side effects of anticancer drugs. However, common challenges of DDS used for cancer treatment, include poor site-specific accumulation, difficulties in entering the tumor micro environment, poor metastases and treatment efficiency. In this context, non-invasive cancer treatment approaches, with or without DDS, involving the use of light, heat, magnetic field, electrical field and ultrasound appears to be very attractive. These approaches can potentially improve treatment efficiency, reduce recovery time, eliminate infections and scar formation. In this review we focus on the effects of magnetic fields and ultrasound on cancer cells and their application for cancer treatment in the presence of drugs or DDS.
COMPLETE STUDY
LUNG CANCER TREATMENT
LUNG CANCER AND PEMF TREATMENT
Daily PEMF was found to retard angiogenesis and growth of a human breast cancer xenograph causing the tumor to develop proportionately larger areas of necrosis and hypoxia and smaller areas of proliferatively active cancer cells.
Pulsed Electromagnetic Field Therapy, abbreviated PEMF, is a very simple, painless, and non-invasive way to address abnormal resonance in cancer cells. In addition, PEMF has been shown to assist in making cell membranes more permeable.
ABSTRACT
Lung cancer is the leading cause of cancer deaths and the overall 5-year survival rate is less than 17%. Hyperthermia is an alternative approach for the treatment of lung cancer and is associated with fewer side effects. We employed ironoxide nanoparticles in inducing localized hyperthermia in lung cancer cells using a pulsed electromagnetic field (PEMF). We synthesized, characterized and determined the uptake of dipeptide-coated iron oxide nanoparticles. Further, their ability in inducing localized hyperthermia in PEMF on lung cancer cells was assessed. Results showed nanoparticles are non-cytotoxic and showed enhanced cellular uptake in lung cancer cells. In vivo studies in nude mice lung tumor xenografts confirmed the presence in the tumors. Lung cancer cells pretreated with dipeptide-coated magnetic nanoparticles upon PEMF exposure induced cell death.
COMPLETE STUDY
PANCREATIC CANCER TREATMENT
PANCREATIC CANCER AND PEMF TREATMENT
By inducing a mild electrical magnetic current into damaged cells, PEMF therapy slows or stops the release of pain and inflammatory mediators, increases blood flow of the cells, and re-establishes normal cell interaction. With reduced inflammation, pain decreases, energy increases, and faster tissue healing occurs.”
Pulsed Electromagnetic Field Therapy, abbreviated PEMF, is a very simple, painless, and non-invasive way to address abnormal resonance in cancer cells. In addition, PEMF has been shown to assist in making cell membranes more permeable.
ABSTRACT
Cancer is one of the most common causes of death worldwide. Available treatments are associated with numerous side effects and only a low percentage of patients achieve complete remission. Therefore, there is a strong need for new therapeutic strategies. In this regard, pulsed electromagnetic field (PEMF) therapy presents several potential advantages including non‐invasiveness, safety, lack of toxicity for non‐cancerous cells, and the possibility of being combined with other available therapies. Indeed, PEMF stimulation has already been used in the context of various cancer types including skin, breast, prostate, hepatocellular, lung, ovarian, pancreatic, bladder, thyroid, and colon cancer in vitro and in vivo. At present, only limited application of PEMF in cancer has been documented in humans. In this article, we review the experimental and clinical evidence of PEMF therapy discussing future perspectives in its use in oncology.
COMPLETE STUDY
PEMF CANCER RESEARCH
The specific claim, supported by the described in vivo studies, is that all treated groups showed slower tumor growth rate if compared with untreated control group, confirming that PEMF therapy can modulate the physiology and electrochemistry of cancer cells and influence cell membrane systems and mitosis.
Mechanisms and therapeutic effectiveness of pulsed electromagnetic field therapy in oncology
Pulsed Electromagnetic Fields and their Role in Breast Cancer Impedance